"14-3-3σ抗体上海远慕生物有限公司专业供应各种生物试剂、生化试剂，单抗多抗抗体，各种二抗，标 记抗体，抗体抗原，蛋白多肽产品，Elisa酶联免疫试剂盒，各种细胞株、细胞系，各种 动物血清，金标试剂盒，放免试剂盒，培养基，毒素 的产品， 实时价格和产品详细说明 ，敬请来电咨询！

产品名称：14-3-3σ抗体
产品货号：YM-H8984-X
14-3-3σ抗体多克隆抗体的制备一般包括以下几个步骤：
1、制备抗原。
2、选择实验动物。
3、动物免疫。
4、试取血进行测试，看看是否成功免疫
5、如果成功免疫，杀死实验动物，采集全部血清。
6、纯化出抗体。
7、鉴定抗体。包括纯度以及特异性。

14-3-3σ抗体相关产品：

14-3-3σ抗体产品WB实验中的对照系统：
he chromogenic reaction （Note: two anti generally have two kinds ofcommonly used marker enzyme, HRP and AP, chromogenic substrate kit andits corresponding to DAB and BCIP/NBT, the general use ofchemiluminescence lysate.） Westernbloting is the first to transfer afterelectrophoresis separation of proteins from the gel onto a NC membrane,there are generally two ways: capillary blotting and electrophoreticblotting. Westernbloting is a common method used to detect the protein,through the immune reaction of antigen antibody can be quantitativelymeasured the relative molecular weight of antibody, and the relativeabundance of known antigen or antibody and the relation between. Atpresent, it is applied in many fields, detection of infectious diseasesand autoimmune diseases, allergies, etc. in clinic, in antibodyproduction field it with ELISA, ICC, IHC, IFA and other immunetechnology has become the main means of engaging a screening ofmonoclonal antibody production.
WB对照系统
阳性对照：最好有标准品，或阳性血清、阳性上清。
阴性对照：测血时用相应小鼠未免疫血清，测培养上清，用无克隆培养上清。
空白对照：不加一抗，用1%BSA代替。
无关对照（替代对照）：用无关项目一抗，或无关项目的抗体。
"